Revista: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000391518 |
ISSN: | 1516-8913 |
Autors: | Kaur, Hardeep1 Kumar, Sachin1 Verma, Neelam1 |
Institucions: | 1Punjabi University, Department of Biotechnology, Patiala, Punjab. India |
Any: | 2014 |
Període: | Jul-Ago |
Volum: | 57 |
Número: | 4 |
Paginació: | 613-619 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 - phenanthroline and sodium potassium tartarate. Validation of the developed biosensors was carried out with spiked milk samples |
Disciplines | Química |
Paraules clau: | Química de alimentos, Leche, Análisis de alimentos, Plomo, Biosensores, Colorimetría, Potenciometría, Ureasa |
Keyword: | Chemistry, Food chemistry, Milk, Food analysis, Lead, Biosensors, Colorimetry, Potentiometry, Urease |
Text complet: | Texto completo (Ver HTML) |