Revista: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000391517 |
ISSN: | 1516-8913 |
Autors: | Song, Junying1 Liu, Hongxia1 Wang, Li1 Dai, Jun1 Liu, Yan1 Liu, Hui1 Zhao, Genhai1 Zheng, Zhiming1 Wang, Peng1 |
Institucions: | 1Chinese Academy of Sciences, Key Laboratory of Ion Beam Bioengineering, Hefei, Anhui. China |
Any: | 2014 |
Període: | Jul-Ago |
Volum: | 57 |
Número: | 4 |
Paginació: | 606-612 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66-fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1was increased 55% and reached 3.593±0.107 mg/L by using the Plackett-Burman and Box-Behnken designs to optimize the fermentation medium. The optimal fermentation culture medium was composed of (g/L) glycerol 69.6, sucrose 34.5, K2HPO4 4.0, peptone 20, yeast extract 25 and fermented at 37 °C and 150 rpm for 72 h. The results showed that the NTG and low energy ion beam implantation mutations and optimizing fermentation medium were effective methods to enhance vitamin K2 production |
Disciplines | Química |
Paraules clau: | Fermentaciones, Química farmacéutica, Biotecnología, Mutantes, Bacillus subtilis, Bioproducción, Vitamina K2 |
Keyword: | Chemistry, Fermentation, Medicinal chemistry, Biotechnology, Fermentations, Mutants, Bacillus subtilis, Bioproduction, Vitamin K2 |
Text complet: | Texto completo (Ver HTML) |