| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000405544 |
| ISSN: | 0074-0276 |
| Authors: | Santos, Paula Fernanda Goncalves dos1 Costa, Elis Regina Dalla2 Ramalho, Daniela M1 Rossetti, Maria Lucia2 Barcellos, Regina Bones1 Nunes, Luciana de Souza3 Esteves, Leonardo Souza2 Rodenbusch, Rodrigo2 Anthony, Richard M4 Bergval, Indra4 Sengstake, Sarah4 Viveiros, Miguel5 Kritski, Afranio1 Oliveira, Martha M1 |
| Institutions: | 1Royal Tropical Institute, KIT Biomedical Research, Amsterdam. Países Bajos |
| Year: | 2017 |
| Season: | Jun |
| Volumen: | 112 |
| Number: | 6 |
| Pages: | 396-403 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | To cope with the emergence of multidrug-resistant tuberculosis (MDR-TB), new molecular methods that can routinely be used to screen for a wide range of drug resistance related genetic markers in the Mycobacterium tuberculosis genome are urgently needed. OBJECTIVE To evaluate the performance of multiplex ligaton-dependent probe amplification (MLPA) against Genotype® MTBDRplus to detect resistance to isoniazid (INHr) and rifampicin (RIFr). METHOD 96 culture isolates characterised for identification, drug susceptibility testing (DST) and sequencing of rpoB, katG, and inhA genes were evaluated by the MLPA and Genotype®MTBDRplus assays. RESULTS With sequencing as a reference standard, sensitivity (SE) to detect INHr was 92.8% and 85.7%, and specificity (SP) was 100% and 97.5%, for MLPA and Genotype®MTBDRplus, respectively. In relation to RIFr, SE was 87.5% and 100%, and SP was 100% and 98.8%, respectively. Kappa value was identical between Genotype®MTBDRplus and MLPA compared with the standard DST and sequencing for detection of INHr [0.83 (0.75-0.91)] and RIFr [0.93 (0.88-0.98)]. CONCLUSION Compared to Genotype®MTBDRplus, MLPA showed similar sensitivity to detect INH and RIF resistance. The results obtained by the MLPA and Genotype®MTBDRplus assays indicate that both molecular tests can be used for the rapid detection of drug-resistant TB with high accuracy. MLPA has the added value of providing information on the circulating M. tuberculosis lineages |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Microbiología, Tuberculosis, Resistencia a fármacos, Isoniazida, Rifampicina, Marcadores genéticos, Mycobacterium tuberculosis |
| Keyword: | Medicine, Microbiology, Pharmacology, Tuberculosis, Drug resistance, Isoniazid, Rifampicin, Genetic markers, Mycobacterium tuberculosis |
| Full text: | Texto completo (Ver HTML) |
