| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000405558 |
| ISSN: | 0074-0276 |
| Authors: | Mello, Cíntia da Silva1 Valente, Ligia Maria Marino2 Wolff, Thiago2 Lima-Junior, Raimundo Sousa1 Fialho, Luciana Gomes1 Marinho, Cintia Ferreira1 Azeredo, Elzinandes Leal1 Oliveira-Pinto, Luzia Maria1 Pereira, Rita de Cassia Alves3 Siani, Antonio Carlos4 Kubelka, Claire Fernandes1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Quimica, Rio de Janeiro. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Agroindustria Tropical, Fortaleza, Ceara. Brasil 4Fundacao Oswaldo Cruz, Instituto de Tecnologia em Farmacos, Rio de Janeiro. Brasil |
| Year: | 2017 |
| Season: | Jun |
| Volumen: | 112 |
| Number: | 6 |
| Pages: | 458-468 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection and in immunological parameters associated with in vivo physiopathological features. METHODS Chemical profiles from UGB or UGL were compared in thin layer chromatography and 1H nuclear magnetic resonance using flavonoid compounds and a pentacyclic oxindole alkaloid-enriched fraction as references. DENV-2-infected hepatocytes (Huh-7) were treated with extracts. Cell viability, DENV antigens and immunological factors were detected by enzyme-linked immunosorbent assay (ELISA) or flow cytometry. FINDINGS The UGL mainly differed from UGB by selectively containing the flavonoid kaempferitrin. UGB and UGL improved hepatocyte viability. Both extracts reduced intracellular viral antigen and inhibited the secretion of viral non-structural protein (NS1), which is indicative of viral replication. Reduction in secretion of macrophage migration inhibitory factor was achieved by UGB, of interleukin-6 by UGL, and of interleukin-8 by both UGB and UGL. MAIN CONCLUSIONS The U. guianensis extracts presented, antiviral and immunomodulatory effects for DENV and possibly a hepatocyte-protective activity. Further studies may be performed to consider these products as potential candidates for the development of an herbal product for the future treatment of dengue |
| Disciplines: | Medicina, Química |
| Keyword: | Farmacología, Microbiología, Fitoquímica, Dengue, Plantas medicinales, Inmunomodulación, Actividad antiviral, Virus del dengue, Uncaria guianensis, Rubiaceae |
| Keyword: | Medicine, Chemistry, Microbiology, Pharmacology, Phytochemistry, Dengue, Medicinal plants, Immunomodulation, Antiviral activity, Dengue virus, Uncaria guianensis, Rubiaceae |
| Full text: | Texto completo (Ver HTML) |
