Journal: | Genetics and molecular biology |
Database: | PERIÓDICA |
System number: | 000401316 |
ISSN: | 1415-4757 |
Authors: | Oliveira, Daniel S1 Gomes, Tiago M.F.F1 Loreto, Elgion L.S1 |
Institutions: | 1Universidade Federal de Santa Maria, Centro de Ciencias Naturais e Exatas, Santa Maria, Rio Grande do Sul. Brasil |
Year: | 2016 |
Season: | Dic |
Volumen: | 39 |
Number: | 4 |
Pages: | 611-625 |
Country: | Brasil |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, aplicado |
English abstract | The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two protein-coding and two ribosomal genes, along with the 16 tRNA genes. This gene order is different from the pattern described for Ibalia leucospoides (Ibaliidae, Cynipoidea), suggesting that this particular gene order can be variable among Cynipoidea superfamily members. A maximum likelihood phylogenetic analysis of the main groups of Apocrita was performed using amino acid sequence of 13 protein-coding genes, showing monophyly for the Cynipoidea superfamily within the Hymenoptera phylogeny |
Disciplines: | Biología |
Keyword: | Genética, Insectos, Avispas parasitoides, Mitocondrias, Secuencia genómica, Leptopilina boulardi, Hymenoptera |
Keyword: | Biology, Genetics, Insects, Parasitoid wasps, Mitochondria, Genomic sequence, Leptopilina boulardi, Hymenoptera |
Full text: | Texto completo (Ver HTML) |