| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000366027 |
| ISSN: | 0327-9545 |
| Authors: | Zukas, Randi1 Chang, Alex J1 Rice, Marian2 Springer, Amy L1 |
| Institutions: | 1Amherst College, Department of Biology, Amherst, Massachusetts. Estados Unidos de América 2Mount Holyoke College, Department of Biological Sciences, South Hadley, Massachusetts. Estados Unidos de América |
| Year: | 2012 |
| Season: | Dic |
| Volumen: | 36 |
| Number: | 3 |
| Pages: | 133-141 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the α heavy chain, and IC138, an intermediate chain, cause severe motility defects including immotility. To determine if motility defects result from structural disruption of the axoneme, we used two different flagellar preparations to carefully examine axoneme structure in these strains using transmission electron microscopy (TEM). Our analysis showed that inner arm dynein size, axoneme structural integrity and fixed central pair orientation are not significantly different in either knockdown culture when compared to control cultures. These results support the idea that immotility in knockdowns affecting DNAH10 or IC138 results from loss of dynein-f function rather than from obvious structural defects in the axoneme |
| Disciplines: | Biología |
| Keyword: | Biología celular, Protozoarios, Morfología celular, Trypanosoma brucei, Flagelos, Axonema, Microscopio electrónico de transmisión, Motilidad |
| Keyword: | Biology, Cell biology, Protozoa, Cell morphology, Trypanosoma brucei, Flagella, Axoneme, Transmission electron microscopy, Motility |
| Full text: | Texto completo (Ver PDF) |
