| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000366028 |
| ISSN: | 0327-9545 |
| Authors: | Frescura, Viviane dal-Souto1 Laughinghouse, Haywood Dail2 Canto-Dorow, Thais Scotti do1 Tedesco, Solange Bosio1 |
| Institutions: | 1Universidade Federal de Santa Maria, Centro de Ciencias da Naturais e Exatas, Santa Maria, Rio Grande do Sul. Brasil 2University of Liege, Centre for Protein Engineering, Lieja. Bélgica |
| Year: | 2012 |
| Season: | Dic |
| Volumen: | 36 |
| Number: | 3 |
| Pages: | 143-145 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Nota breve o noticia |
| Approach: | Experimental, analítico |
| English abstract | Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of P. paniculata using the Alexander’s stain, which proved the most adequate method to estimate pollen viability |
| Disciplines: | Biología |
| Keyword: | Anatomía e histología, Botánica, Polen, Polygala paniculata, Polygalaceae, Técnicas de tinción, Viabilidad |
| Keyword: | Biology, Anatomy and histology, Botany, Pollen, Polygala paniculata, Polygalaceae, Staining techniques, Viability |
| Full text: | Texto completo (Ver PDF) |
