| Revista: | The journal of venomous animals and toxins including tropical diseases |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298799 |
| ISSN: | 1678-9199 |
| Autores: | Oliveira, J.S1 Pires-Junior, O.R2 Morales, R.A.V Bloch-Junior, C3 Schwartz, C.A Freitas, J.C |
| Instituciones: | 1Universidade de Sao Paulo, Instituto de Biociencias, Sao Paulo. Brasil 2Universidade de Brasilia, Departamento de Ciencias Fisiologicas, Brasilia, Distrito Federal. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Centro Nacional de Pesquisa de Recursos Geneticos e Biotecnologia, Brasilia, Distrito Federal. Brasil |
| Año: | 2003 |
| Volumen: | 9 |
| Número: | 1 |
| Paginación: | 76-88 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | In Brazil, where puffer fish are considered poisonous, there are few documented cases on human consumption and consequent poisoning. In this study, toxicity of two puffer fish species from the Brazilian coast was examined. Specimens of Sphoeroides spengleri and Lagocephalus laevigatus were caught in São Sebastião Channel (North coast of São Paulo State, Brazil) between January 1996 and May 1997. Acidic ethanol extracts from muscle and skin plus viscera were tested for mice acute toxicity using the standard method of Kawabata. Polar extracts of S. spengleri showed high toxicity up to 946 MU/g. Extracts from L. laevigatus showed very low levels of toxicity, never exceeding 1.7 MU/g. All extracts from both species blocked amielinic nerve fiber evoked impulses of crustacean legs; this effect reverted on washing similar to the standard tetrodotoxin TTX. The aqueous extract solutions were partially purified using an ionic exchange column (Amberlit GC-50) followed by treatment with activated charcoal (Norit-A). The presence of TTX and their analogs in the semi-purified extracts were confirmed by HPLC and mass spectrometry (MALDI-TOF) |
| Disciplinas: | Medicina, Biología |
| Palabras clave: | Toxicología, Peces, Tetrodotoxina, Envenenamiento, Lagocephalus laevigatus, Sphoeroides spengleri |
| Keyword: | Medicine, Biology, Toxicology, Fish, Tetrodotoxin, Envenomation, Lagocephalus laevigatus, Sphoeroides spengleri |
| Texto completo: | Texto completo (Ver HTML) |
