Profile of antimicrobial resistance of bacteria isolated from cockroaches (Periplaneta americana) in a Brazilian health care institution



Título del documento: Profile of antimicrobial resistance of bacteria isolated from cockroaches (Periplaneta americana) in a Brazilian health care institution
Revista: The brazilian journal of infectious diseases
Base de datos: PERIÓDICA
Número de sistema: 000291027
ISSN: 1413-8670
Autores: 1
2


Instituciones: 1Universidade Federal de Goias, Goiania, Goias. Brasil
2Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo. Brasil
Año:
Periodo: Feb
Volumen: 10
Número: 1
Paginación: 26-32
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Caso clínico
Resumen en inglés Cockroaches carry microorganisms to sterilized materials, equipment and non-contaminated food in hospitals and in homes. Hence, they are regarded as major microbial vectors. We isolated and identified microorganisms from living cockroaches captured from a Brazilian health care institution and we determined the antimicrobial susceptibility profile of the isolates. Following collection, cockroaches were immobilized at 4ºC for 10 to 20 minutes, immersed in 0.8% saline solution and then homogenized. Next, the suspension was seeded in culture media: brain and heart agar infusion, MacConkey agar, Naito agar, and they were incubated for up to 48 hours at 37ºC. Sabouraud agar cultures were incubated at ambient temperature. The microbial colonies were analyzed with a stereomicroscope to count and phenotypically identify the colony-forming units (cfu). The discdiffusion method was used to determine the profile of susceptibility to antimicrobials. Among the 103 cockroaches analyzed, fungi were found in 97%, enterobacteria in 74.6% and coagulase-negative staphylococci (CNS) in 25.40%. Among the enterobacteria, 96% were resistant to gentamicin, 84% to ampicillin, 75.3% to caphalothin, 66.7% to ampicillin-sulbactam, 50% to aztreonam and 30% to chloramphenicol. Among the CNS, 61% were resistant to oxacillin. We concluded that cockroaches carry organisms associated with nosocomial infections and that are resistant to antimicrobials. This fact contributes to the epidemiological chain, complicating therapeutics, and consequently, medical costs ..
Disciplinas: Biología,
Medicina
Palabras clave: Insectos,
Hospitales,
Microbiología,
Salud pública,
Cucarachas,
Microorganismos,
Infección hospitalaria,
Resistencia antimicrobiana
Keyword: Biology,
Medicine,
Insects,
Hospitals,
Microbiology,
Public health,
Cockroaches,
Microorganisms,
Nosocomial infections,
Antimicrobial resistance
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