Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil



Título del documento: Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil
Revista: The brazilian journal of infectious diseases
Base de datos: PERIÓDICA
Número de sistema: 000290868
ISSN: 1413-8670
Autores: 1


1
Instituciones: 1Instituto Adolfo Lutz, Sao Paulo. Brasil
Año:
Periodo: Dic
Volumen: 8
Número: 6
Paginación: 407-418
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Caso clínico
Resumen en inglés A new monoclonal antibody (5F81A4P1.9), which is specific for subtype 9 antigen of meningococci, was studied. The antibodies were raised against a previously non-typable (NT) serogroup B strain from Brazilian patients and were found to react with the subtype antigen of prototype reference strains for subtype 9 (M982), as well as with those of homologous strains. The subtype 9 epitope was found in 6.8% of serogroup B strains among 602 strains of Neisseria meningitidis case isolates, including representative isolates from Brazilian states. Subtype P1.9 was predominantly related to serogroup B in Brazil among the isolates collected during the N. meningitidis epidemic in 1992. No significant differences were observed in the occurrence of subtype P1.9 among strains isolated from several Brazilian states. Fluorescence-activated cell-sorter analysis showed that 5F81A4 MAb recognized a 46 kDa protein on the surface of a homologous strain of N. meningitidis (B:4:P1.9). These results, in association with a bactericidal activity assay for 5F81A4, and with experimental passive protection in mice, demonstrated the importance of subtype 9 class 1 proteins of N meningitidis in Brazil. Serotyping is essential for the development of vaccination strategies
Disciplinas: Química,
Biología
Palabras clave: Bioquímica,
Bacterias,
Inmunología,
Neisseria meningitidis,
Anticuerpos monoclonales,
Brazilian meningococcus strains,
Serotipificación,
Subtipos
Keyword: Chemistry,
Biology,
Biochemistry,
Bacteria,
Immunology,
Neisseria meningitidis,
Monoclonal antibodies,
Serotyping,
Subtypes
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