Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips



Título del documento: Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips
Revista: The brazilian journal of infectious diseases
Base de datos: PERIÓDICA
Número de sistema: 000291009
ISSN: 1413-8670
Autores: 1
2

3
Instituciones: 1Universidade Estadual do Oeste do Parana, Centro de Ciencias Medicas e Farmaceuticas, Marechal Candido Rondon, Parana. Brasil
2Universidade Estadual de Maringa, Departamento de Analisis Clinicos, Maringa, Parana. Brasil
3Universidade Estadual de Londrina, Hospital Universitario, Londrina, Parana. Brasil
Año:
Periodo: Oct
Volumen: 10
Número: 5
Paginación: 346-351
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Caso clínico
Resumen en inglés The cell surface hydrophobicity and adhesion to abiotic and cellular surfaces was tested in five clinical strains of Acinetobacter baumannii isolated from catheter tips. Biochemical and molecular characteristics of these strains were also studied. Hydrophobicity was characterized by a test for affinity to xylene. Adhesion to abiotic surfaces (polystyrene, formica, latex and glass) was evaluated in Petri plates using the stamp technique. Buccal epithelial cells were used for tests of adhesion to cellular surfaces. Adhesion to the catheter was evaluated by repeatedly rinsing the catheters and rolling them over nutrient agar. Molecular typing of the strains was done by the ERIC-PCR technique. The degree of hydrophobicity of the strains varied from hydrophobic to hydrophilic. All the strains adhered to the cell surfaces and to the catheters, and three of them strongly adhered to latex, polystyrene and formica. Catheter adhesion was reduced by meropenem. We found a direct relationship between the degree of bacterial hydrophobicity and adhesion to the abiotic surfaces, but not with adhesion to cellular surfaces, which suggests that different mechanisms are involved in adherence
Disciplinas: Química,
Biología
Palabras clave: Bioquímica,
Bacterias,
Biología celular,
Acinetobacter baumannii,
Hidrofobicidad,
Adherencia
Keyword: Chemistry,
Biology,
Biochemistry,
Bacteria,
Cell biology,
Acinetobacter baumannii,
Hydrophobicity,
Adherence
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