| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446395 |
| ISSN: | 0074-0276 |
| Autores: | Herlinger, Alice Laschuk1 Monteiro, Fábio Luís Lima1 D’arc, Mirela1 Moreira, Filipe Romero Rebello1 Westgarth, Harrison James1 Galliez, Rafael Mello2 Mariani, Diana1 Costa, Luciana Jesus da3 Almeida, Luiz Gonzaga Paula de4 Voloch, Carolina Moreira1 Melo, Adriana Suely de Oliveira6 Aguiar, Renato Santana de7 Santos, André Felipe Andrade dos1 Castiñeiras, Terezinha Marta Pereira Pinto8 Vasconcelos, Ana Tereza Ribeiro de4 João-Filho, Esaú Custódio2 Escosteguy, Claudia Caminha2 Ferreira-Junior, Orlando da Costa1 Tanuri, Amilcar1 Higa, Luiza Mendonça1 |
| Instituciones: | 1Universidade Federal do Rio de Janeiro, Instituto de Biologia, Rio de Janeiro. Brasil 2Hospital Federal dos Servidores do Estado do Rio de Janeiro, Rio de Janeiro. Brasil 3Universidade Federal do Rio de Janeiro, Instituto de Microbiologia "Paulo de Goes", Rio de Janeiro. Brasil 4Laboratorio Nacional de Computacao Cientifica, Laboratorio de Bioinformática, Petropolis, Rio de Janeiro. Brasil 5Universidade Federal do Rio de Janeiro, Rio de Janeiro. Brasil 6Instituto de Pesquisa Professor Amorim Neto, Campina Grande, Paraiba. Brasil 7Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil 8Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Rio de Janeiro. Brasil |
| Año: | 2021 |
| Volumen: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | BACKGROUND During routine Coronavirus disease 2019 (COVID-19) diagnosis, an unusually high viral load was detected by reverse transcription real-time polymerase chain reaction (RT-qPCR) in a nasopharyngeal swab sample collected from a patient with respiratory and neurological symptoms who rapidly succumbed to the disease. Therefore we sought to characterise the infection. OBJECTIVES We aimed to determine and characterise the etiological agent responsible for the poor outcome. METHODS Classical virological methods, such as plaque assay and plaque reduction neutralisation test combined with amplicon-based sequencing, as well as a viral metagenomic approach, were performed to characterise the etiological agents of the infection. FINDINGS Plaque assay revealed two distinct plaque phenotypes, suggesting either the presence of two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains or a productive coinfection of two different species of virus. Amplicon-based sequencing did not support the presence of any SARS-CoV-2 genetic variants that would explain the high viral load and suggested the presence of a single SARS-CoV-2 strain. Nonetheless, the viral metagenomic analysis revealed that Coronaviridae and Herpesviridae were the predominant virus families within the sample. This finding was confirmed by a plaque reduction neutralisation test and PCR. MAIN CONCLUSIONS We characterised a productive coinfection of SARS-CoV-2 and Herpes simplex virus 1 (HSV-1) in a patient with severe symptoms that succumbed to the disease. Although we cannot establish the causal relationship between the coinfection and the severity of the clinical case, this work serves as a warning for future studies focused on the interplay between SARS-CoV-2 and HSV-1 coinfection and COVID-19 severity |
| Disciplinas: | Medicina |
| Palabras clave: | Virus, Microbiología, COVID-19, Alfa-herpesvirus humano 1, Coinfecciones |
| Keyword: | Virus, Microbiology, COVID-19, Human alphaherpesvirus 1, Coinfections |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
