Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
Título del documento: Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000351816
ISSN: 0074-0276
Autores: 1
1
1
1
2
1
Instituciones: 1Instituto Oswaldo Cruz, Departamento de Bioquimica e Biologia Molecular, Rio de Janeiro. Brasil
2Instituto Evandro Chagas, Secao de Parasitologia, Belem, Para. Brasil
Año:
Periodo: Jun
Volumen: 102
Número: 4
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Visceral leishmaniasis (VL) is a serious tropical disease that affects approximately 500 thousand people worldwide every year. In the Americas, VL is caused by the parasite Leishmania (Leishmania) infantum chagasi mainly transmitted by the bite of the sand fly vector Lutzomyia longipalpis. Despite recent advances in the study of interaction between Leishmania and sand flies, very little is known about sand fly protein expression profiles. Understanding how the expression of proteins may be affected by blood feeding and/or presence of parasite in the vector's midgut might allow us to devise new strategies for controlling the spread of leishmaniasis. In this work, we report the characterization of a vacuolar ATPase subunit C from L. longipalpis by screening of a midgut cDNA library with a 220 bp fragment identified by means of differential display reverse transcriptase-polymerase chain reaction analysis. The expression of the gene varies along insect development and is upregulated in males and bloodfed L. longipalpis, compared to unfed flies
Disciplinas: Biología
Palabras clave: Genética,
Insectos,
Expresión génica,
Expresión diferencial,
Metaciclogénesis,
V-ATPasa,
Lutzomyia longipalpis,
Vectores biológicos,
Leishmaniasis
Keyword: Biology,
Genetics,
Insects,
Gene expression,
Differential expression,
Metacyclogenesis,
V-ATPase,
Lutzomyia longipalpis,
Biological vectors,
Leishmaniasis
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