| Revista: | Maderas : ciencia y tecnología |
| Base de datos: | |
| Número de sistema: | 000534453 |
| ISSN: | 0718-221X |
| Autores: | Verick Purba, Brandon Aristo1 Sunarti, Sri2 Lukmandaru, Ganis1 |
| Instituciones: | 1Universitas Gadjah Mada Faculty of Forestry Department of Forest Products Technology, Bulaksumur Yogyakarta. Indonesia 2Center for Forest Biotechnology and Tree Improvement, Purwobinangun Yogyakarta. Indonesia |
| Año: | 2021 |
| Volumen: | 23 |
| País: | Chile |
| Idioma: | Inglés |
| Resumen en inglés | Breeding strategy of Acacia hybrid is being developed by Center for Forest Biotechnology and Tree Improvement Indonesia and has produced three superior clones in growth (Clone 16, 25, and 44). Understanding the extractives of the new clones might determine future development steps to improve its resistance to diseases especially heartrot. The objective of this study was to investigate the extractive content in three radial directions (SW = sapwood; OHW = outer heartwood; IHW = inner heartwood); total phenolic, flavonoid, flavanol contents (colorimetric assay); and antioxidant activity (1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay). Extractions were done with three different solvents in successive (n-hexane = H; methanol = M; hot water = W), yielded 0,69 % - 1,70 %; 1,51 % - 10,86 %; and 0,51 % - 1,16 % of extractive contents, respectively. The total phenolic content (TPC) from TPC-H, TPC-M, and TPC-W ranged between 3,68 mg of gallic acid equivalent (GAE)/g - 10,41 mg GAE/g; 76,83 mg GAE/g - 448,35 mg GAE/g; and 43,28 mg GAE/g - 198,92 mg GAE/g, respectively; the total flavonoid content (TFC) from TFC-H, TFC-M, and TFC-W between 4,23 mg of quercetin equivalent (QE)/g - 41,51 mg QE/g; 29,55 mg QE/g - 133,71 mg QE/g; and 7,70 mg QE/g - 29,37 mg QE/g, respectively; total flavanol content (TVC) from TVC-H, TVC-M, and TVC-W ranged between 28,74 mg of catechin equivalent (CE)/g - 66,90 mg CE/g; 83,39 mg CE/g - 247,18 mg CE/g; and 7,08 mg CE/g - 29,21 mg CE/g, respectively. Furthermore, the antioxidant activity was found to be significantly affected by the radial factor with the strongest activity exhibited by inner heartwood extract with an IC50 value of 255,77 μg/ml (gallic acid IC50 showed a value of 39,00 μg/ml). Among clones, clone 16 was determined to have the highest extractive, total flavonoid as well as flavanol contents. Thus, clone 16 was hypothesized to be more resistance against heart rot disease. |
| Keyword: | Acacia hybrid, Heartwood, Phenolic contents, Sapwood, Wood extractives. |
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