Propagation of Dendrobium aggregatum by green capsule culture



Título del documento: Propagation of Dendrobium aggregatum by green capsule culture
Revista: Lankesteriana
Base de datos: PERIÓDICA
Número de sistema: 000367939
ISSN: 1409-3871
Autores: 1
2
1
Instituciones: 1Government Arts College, Research Department of Botany, Coimbatore, Tamil Nadu. India
2Hindusthan College of Arts & Science, Department of Biotechnology, Coimbatore, Tamil Nadu. India
Año:
Periodo: Ago
Volumen: 12
Número: 2
Paginación: 131-135
País: Costa Rica
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month
Disciplinas: Biología,
Química
Palabras clave: Angiospermas,
Fisiología vegetal,
Bioquímica,
Dendrobium aggregatum,
Protocormos,
Aclimatación,
Cultivo in vitro,
Biotecnología
Keyword: Biology,
Chemistry,
Angiosperms,
Plant physiology,
Biochemistry,
Dendrobium aggregatum,
Protocorms,
Acclimatization,
In vitro culture,
Biotechnology
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