| Revista: | Jornal brasileiro de patologia e medicina laboratorial |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000297235 |
| ISSN: | 1676-2444 |
| Autores: | Alves, Maria Teresa de Seixas1 Roman, Leonor Cristina Manoja |
| Instituciones: | 1Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Ribeirao Preto, Sao Paulo. Brasil |
| Año: | 2005 |
| Periodo: | Feb |
| Volumen: | 41 |
| Número: | 1 |
| Paginación: | 43-49 |
| País: | Brasil |
| Idioma: | Portugués |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | The effects of fixation time in buffered formaline at 10% on different methods of antigenic site retrieval induced by heat (HIER) for the detection of the expression of antibodies anti-PCNA (nuclear proliferation antigen) and anti-AE1 and AE3 (citokeratins) were studied. Specimens with cross section of 0,5 cm² from five Tonsils from ellective tonsillectomies done at Hospital São Paulo UNIFESP/EPM were fixed for six, 12, 18, 24 and 48 hours and embedded in paraffin for the production of 4mum sections. For HIER was used a steamer, microwave oven and pressure cooker. The level of PCNA expression was assessed by the ratio stained nuclei: total number of nuclei. The staining intensity was assessed by using Corel Photo Paint 9 and UT Morph 2.0 softwares. The present study has shown that: 1) for the detection of anti-PCNA, the use of a microwave oven provided the best results; 2) for the detection of anti-PCNA, longer fixation periods, specially after 24 hours, causes the staining intensity to decrease; 3) for the detection of AE1 and AE3, all procedures used provided equivalent levels of staining |
| Resumen en portugués | Em relação ao parâmetro intensidade de coloração para AE1AE3 nenhum dos métodos foi superior. A metodologia estudada para a análise semiquantitativa na intensidade da reação coincidiu com os resultados obtidos na avaliação criteriosa de cada uma das lâminas |
| Disciplinas: | Medicina |
| Palabras clave: | Inmunología, Inmunohistoquímica, Técnicas de laboratorio, Antígenos, Recuperación |
| Keyword: | Medicine, Immunohistochemistry, Laboratory techniques, Antigens, Recuperation, Immunology |
| Texto completo: | Texto completo (Ver HTML) |
