| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459275 |
| ISSN: | 1415-4757 |
| Autores: | Husin, Nurul Arneida1 Rahman, Sadequr2 Karunakaran, Rohini3 Bhore, Subhash Janardhan1 |
| Instituciones: | 1AIMST University, Faculty of Applied Sciences, Kedah. Malasia 2Monash University Malaysia, School of Science and Tropical Medicine and Biology Platform, Bandar Sunway, Kuala Lumpur. Malasia 3AIMST University, Faculty of Medicine, Kedah. Malasia |
| Año: | 2022 |
| Volumen: | 45 |
| Número: | 4 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | Durian (Durio zibethinus Murr.) fruits are famous for their unique aroma. This study analysed the Durian fruit transcriptome to discover the expression patterns of genes and to understand their regulation. Three developmental stages of Durian fruit, namely, early [90 days post-anthesis (DPA)], mature (120 DPA), and ripen (127 DPA), were studied. The Illumina HiSeq platform was used for sequencing. The sequence data were analysed using four different mapping aligners and statistical methods: CLC Genomic Workbench, HISAT2+DESeq2, Tophat+Cufflinks, and HISAT2+edgeR. The analyses showed that over 110 million clean reads were mapped to the Durian genome, yielding 19,976, 11,394, 17,833, and 24,351 differentially expressed genes during 90-127 days post-anthesis. Many identified differentially expressed genes were linked to the fruit ripening processes. The data analysis suggests that most genes with increased expression at the ripening stage were primarily involved in the metabolism of cofactors and vitamins, nucleotide metabolism, and carbohydrate metabolism. Significantly expressed genes from the young to mature stage were mainly associated with carbohydrate metabolism, amino acid metabolism, and cofactor and vitamin metabolism. The transcriptome data will serve as a foundation for understanding Durian fruit development-specific genes and could be helpful in fruit’s trait improvement |
| Disciplinas: | Biología |
| Palabras clave: | Genética, Botánica, Durio zibethinus, Frutos, Transcriptoma, Maduración del fruto |
| Keyword: | Genetics, Botany, Durio zibethinus, Fruits, Transcriptome, Fruit ripening |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
