| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000371757 |
| ISSN: | 1415-4757 |
| Autores: | Ding, Bo1 Wang, Junbin2 Song, Na3 Li, Ming1 Cheng, Qiaolin1 Huang, Guozhong1 Guo, Yaolin1 Yang Fu1 Xie, Chaojie3 Sun, Qixin3 Xie, Xiaodong1 |
| Instituciones: | 1Tianjin Agricultural University, Tianjin-Bristol Research Centre for the Effects of Environmental Change on Crops, Tianjin. China 2Tianjin Agricultural University, Department of Basic Science, Tianjin. China 3China Agricultural University, State Key Laboratory for Agrobiotechnology, Beijing. China |
| Año: | 2014 |
| Periodo: | Mar |
| Volumen: | 37 |
| Número: | 1 |
| Paginación: | 73-80 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | WRKY transcription factors have been extensively characterized in the past 20 years, but in wheat, studies onWRKY genes and their function are lagging behind many other species. To explore the function of wheat WRKY genes, we identified a TaWRKY68 gene from a common wheat cultivar. It encodes a protein comprising 313 amino acids which harbors 19 conserved motifs or active sites. Gene expression patterns were determined by analyzing microarray data of TaWRKY68 in wheat and of orthologous genes from maize, rice and barley using Genevestigator. TaWRKY68 orthologs were identified and clustered using DELTA-BLAST and COBALT programs available at NCBI. The results showed that these genes, which are expressed in all tissues tested, had relatively higher levels in the roots and were up-regulated in response to biotic stresses. Bioinformatics results were confirmed by RT-PCR experiments using wheat plants infected by Agrobacterium tumefaciens and Blumeria graminis, or treated with Deoxynivalenol, a Fusarium graminearum-induced mycotoxin in wheat or barley. In summary,TaWRKY68 functions differ during plant developmental stages and might be representing a hub gene function in wheat responses to various biotic stresses. It was also found that including data from major cereal genes in the bioinformatics analysis gave more accurate and comprehensive predictions of wheat gene functions |
| Disciplinas: | Agrociencias |
| Palabras clave: | Fitotecnia, Gramíneas, Genética, Trigo, Mejoramiento genético, Genes |
| Keyword: | Agricultural sciences, Crop husbandry, Gramineae, Genetics, Wheat, Genes, Genetic improvement |
| Texto completo: | Texto completo (Ver PDF) |
