| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000337170 |
| ISSN: | 1415-4757 |
| Autores: | Chae, Song Wha1 Jee, Bo Keun1 Lee, Joo Yong1 Han, Chang Whan2 Jeon, Yang-Whan3 Lim, Young4 Lee, Kweon-Haeng5 Rha, Hyoung Kyun1 Chae, Gue-Tae6 |
| Instituciones: | 1The Catholic University of Korea, Neuroscience Genome Research Center, Seúl. Corea del Sur 2The Catholic University of Korea, College of Medicine, Seúl. Corea del Sur 3The Catholic University of Korea, Department of Psychiatry, Incheon. Corea del Sur 4The Catholic University of Korea, Department of Occupational and Environmental Medicine, Seúl. Corea del Sur 5The Catholic University of Korea, Department of Pharmacology, Seúl. Corea del Sur 6The Catholic University of Korea, Institute of Hansen’s Disease, Seúl. Corea del Sur |
| Año: | 2008 |
| Periodo: | Dic |
| Volumen: | 31 |
| Número: | 4 |
| Paginación: | 815-823 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Human bone marrow-derived mesenchymal stem cells (hMSCs) have the capacity to differentiate into osteoblasts during osteogenesis. Several studies attempted to identify osteogenesis-related genes in hMSCs. Although HOX genes are known to play a pivotal role in skeletogenesis, their function in the osteogenesis of hMSCs has not yet been investigated in detail. Our aim was to characterize the expression of 37 HOX genes by multiplex RT-PCR to identify the ones most probably involved in osteogenic differentiation. The results showed that the expression patterns of four HOX genes were altered during this process. In particular, the expression levels of HOXC13 and HOXD13 were dramatically changed. Real-time PCR and Western blot analysis were performed in order to further analyze the expression of HOXC13 and HOXD13. The qRT-PCR results showed that transcription of HOXC13 was up-regulated by up to forty times, whereas that of HOXD13 was down-regulated by approximately five times after osteogenic differentiation. The Western blot results for the HOXC13 and HOXD13 proteins also corresponded well with the real-time PCR result. These findings suggest that HOXC13 and HOXD13 might be involved in the osteogenic differentiation of hMSCs |
| Disciplinas: | Medicina |
| Palabras clave: | Hematología, Genética, Biología celular, Células madre, Mesenquima humano, Genes Hox, Diferenciación osteogénica, Diferenciación de células madre, Perfil génico |
| Keyword: | Medicine, Hematology, Genetics, Cell biology, Stem cells, Human mesenchymal, Hox genes, Osteogenic differentiation, Gene profiling |
| Texto completo: | Texto completo (Ver PDF) |
