| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000390257 |
| ISSN: | 1415-4757 |
| Autores: | Ceita, Geruza de Oliveira1 Vilas-Boas, Laurival Antonio2 Castilho, Marcelo Santos3 Carazzolle, Marcelo Falsarella5 Pirovani, Carlos Priminho6 Selbach-Schnadelbach, Alessandra4 Gramacho, Karina Peres7 Ramos, Pablo Ivan Pereira4 Barbosa, Luciana Veiga4 Pereira, Goncalo Amarante Guimaraes5 Goes-Neto, Aristoteles1 |
| Instituciones: | 1Universidade Estadual de Feira de Santana, Laboratorio de Pesquisa em Microbiologia Aplicada, Feira de Santana, Bahia. Brasil 2Universidade Estadual de Londrina, Centro de Ciencias Biologicas, Londrina, Parana. Brasil 3Universidade Federal da Bahia, Salvador, Bahia. Brasil 4Universidade Federal da Bahia, Laboratorio de Biologia Molecular, Salvador, Bahia. Brasil 5Universidade Estadual de Campinas, Campinas, Sao Paulo. Brasil 6Universidade Estadual de Santa Cruz, Ilheus, Bahia. Brasil 7Comissao Executiva do Plano da Lavoura Cacaueira, Centro de Pesquisas do Cacau, Ilheus, Bahia. Brasil |
| Año: | 2014 |
| Periodo: | Dic |
| Volumen: | 37 |
| Número: | 4 |
| Paginación: | 683-693 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches’ broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14 -demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed.ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea |
| Disciplinas: | Biología, Agrociencias |
| Palabras clave: | Genética, Hongos, Fitopatología, Plantas para uso industrial, Basidiomycota, Ergosterol, Theobroma cacao |
| Keyword: | Biology, Agricultural sciences, Fungi, Genetics, Phytopathology, Plants for industrial use, Basidiomycota, Ergosterol, Theobroma cacao |
| Texto completo: | Texto completo (Ver PDF) |
