| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336240 |
| ISSN: | 0717-3458 |
| Autores: | Lopez, Jose V1 Ledger, Angela2 Santiago Vázquez, Lory Z3 Pop, Mihai4 Sommer, Dan D4 Ranzer, Llanie K3 Feldman, Robert A Russell, G. Kerr3 |
| Instituciones: | 1Nova Southeastern University, Oceanographic Center, Dania Beach, Florida. Estados Unidos de América 2Florida Atlantic University, Harbor Branch Oceanographic Institution, Fort Pierce, Florida. Estados Unidos de América 3Florida Atlantic University, Department of Chemistry and Biochemistry, Boca Raton, Florida. Estados Unidos de América 4University of Maryland, Center for Bioinformatics and Computational Biology, College Park, Maryland. Estados Unidos de América |
| Año: | 2011 |
| Periodo: | Ene |
| Volumen: | 14 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-“housekeeping” genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila Big Brother), catalases, reverse transcriptases, ferritins and various “hypothetical” protein sequences. A total of 591 EST sequences were deposited into GenBank [dbEST: FL512138 - FL512331, GH611838, and HO061755-HO062154]. The results represent proof of concept for enrichment of unique transcripts over housekeeping genes, such as actin or ribosomal genes, which comprised approximately 17% of the total dataset. Due to the gene and sequence diversity of some ESTs, such sequences can find utility as molecular markers in current and future studies of this species and other soft coral biogeography, chemical ecology, phylogenetics, and evolution |
| Disciplinas: | Biología |
| Palabras clave: | Genética, Invertebrados, Biotecnología, Corales, Secuenciamiento génico, Erythropodium caribaeorum, Gorgonia ventalina |
| Keyword: | Biology, Genetics, Invertebrates, Biotechnology, Corals, Gene sequencing, Erythropodium caribaeorum, Gorgonia ventalina |
| Texto completo: | Texto completo (Ver PDF) |
