Polymorphisms of the bovine chemokine receptor-like 1 gene and their associations with meat quality traits in Qinchuan cattle (Bos taurus)
Título del documento: Polymorphisms of the bovine chemokine receptor-like 1 gene and their associations with meat quality traits in Qinchuan cattle (Bos taurus)
Revista: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000357885
ISSN: 0717-3458
Autores: 1
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Instituciones: 1Northwest A&F University, College of Animal Science and Technology, Yangling, Shaanxi. China
Año:
Volumen: 16
Número: 1
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G > C and 762C > T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data. Conclusions: The results showed that the G264C SNP and C762T SNP of bovine CMKLR1 were significantly associated with backfat thickness (BFT) and water holding capacity (WHC), respectively
Disciplinas: Medicina veterinaria y zootecnia
Palabras clave: Bovinos,
Medicina veterinaria,
Mejoramiento genético,
Quimocinas,
Polimorfismo genético,
Calidad de la carne,
Bos taurus
Keyword: Veterinary medicine and animal husbandry,
Bovines,
Veterinary medicine,
Genetic improvement,
Chemokines,
Genetic polymorphism,
Meat quality,
Bos taurus
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