| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000327795 |
| ISSN: | 0717-3458 |
| Autores: | Guzmán, Fanny1 Barberis, Sonia2 Illanes, Andrés3 |
| Instituciones: | 1Pontificia Universidad Católica de Valparaíso, Instituto de Biología, Valparaíso. Chile 2Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, San Luis. Argentina 3Pontificia Universidad Católica de Valparaíso, Escuela de Ingeniería Bioquímica, Valparaíso. Chile |
| Año: | 2007 |
| Volumen: | 10 |
| Número: | 2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective |
| Disciplinas: | Química |
| Palabras clave: | Bioquímica, Péptidos, Proteasas, Síntesis en fase sólida, Síntesis enzimática |
| Keyword: | Chemistry, Biochemistry, Peptides, Proteases, Solid-phase synthesis, Enzymatic synthesis |
| Texto completo: | Texto completo (Ver HTML) |
