Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
Título del documento: Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
Revista: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000336141
ISSN: 0717-3458
Autores: 1
1
2
2
1
Instituciones: 1Instituto Nacional de Tecnología Agropecuaria, Instituto de Biotecnología, Castelar, Buenos Aires. Argentina
2Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires. Argentina
Año:
Periodo: Nov
Volumen: 13
Número: 6
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (ESTs) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDels) and 304 amplified fragment length polymorphisms (AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower
Disciplinas: Biología,
Agrociencias
Palabras clave: Genética,
Biotecnología,
Mejoramiento genético,
Mapeo genético,
Girasol,
Helianthus annuus
Keyword: Biology,
Agricultural sciences,
Genetics,
Biotechnology,
Plant breeding,
Genetic mapping,
Sunflower,
Helianthus annuus
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