Revista: | Electronic journal of biotechnology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000336140 |
ISSN: | 0717-3458 |
Autores: | Gedikli, Serap1 Aytar, Pinar1 Unal, Arzu2 Yamac, Mustafa3 Cabuk, Ahmet3 Kolankaya, Nazif4 |
Instituciones: | 1Eskisehir Osmangazi University, Graduate School of Natural and Applied Sciences, Eskisehir. Turquía 2Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research, Ankara. Turquía 3Eskisehir Osmangazi University, Faculty of Arts and Science, Eskisehir. Turquía 4Hacettepe University, Faculty of Science, Beytepe, Ankara. Turquía |
Año: | 2010 |
Periodo: | Nov |
Volumen: | 13 |
Número: | 6 |
País: | Chile |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched. At first, a culture medium was selected suitable for lacasse production. To increase the production of lacasse using different inducers and to examine the ability of dechlorination, this article focuses on screen lacasse activity of 21 basidiomycetes isolates grown in five culture media. All inducers evaluated influenced lacasse activity positively except for gallic acid, mannitol, and malt extract for studied isolates. Our findings showed that lacasse activity of Trametes versicolor ATCC (200801) when it was induced with wheat bran reached up to 29.08 U ml-1 and was examined the ability of dechlorination of 2,4,5-trichlorophenol (2,4,5-TCP). The parameters including pH, initial substrate concentration, amount of enzyme, period of reaction, and temperature were tested for dechlorination process. Correlation between oxygen consumption and dechlorination processes under the determined optimum conditions was analyzed. Toxicity of 2,4,5-TCP before and after enzymatic treatment was evaluated by Microtox test. The results demonstrated that toxicity of intermediates formed 2,4,5-TCP did not change |
Disciplinas: | Biología |
Palabras clave: | Hongos, Biotecnología, Fermentación, Lacasa, Inductores químicos, Bioproducción, Declorinación, Basidiomycetes, 2,4,5-tricolorofenol |
Keyword: | Biology, Fungi, Biotechnology, Fermentation, Laccase, Chemical inducers, Bioproduction, Dechlorination, 2,4,5-trichlorophenol, Basidiomycetes |
Texto completo: | Texto completo (Ver PDF) |