| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336235 |
| ISSN: | 0717-3458 |
| Autores: | Milosevic, Snezana1 Subotic, Angelina1 Bulajic, Aleksandra2 Djekic, Ivana2 Jevremovic, Sladjana1 Vucurovic, Ana2 Krstic, Branka2 |
| Instituciones: | 1University of Belgrade, Institute for Biological Research Sinisa Stankovic, Belgrado. Serbia 2University of Belgrade, Faculty of Agriculture, Belgrado. Serbia |
| Año: | 2011 |
| Periodo: | Ene |
| Volumen: | 14 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 µM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures |
| Disciplinas: | Agrociencias |
| Palabras clave: | Fitopatología, Plantas ornamentales, Cultivo de tejidos, Virus, Virus del bronceado del tomate, Biotecnología, Impatiens hawkerii |
| Keyword: | Agricultural sciences, Ornamental plants, Phytopathology, Biotechnology, Tissue culture, Tomato spotted wilt virus, Virus, Impatiens hawkerii |
| Texto completo: | Texto completo (Ver HTML) |
