Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and genebank collection by the neutral and functional genomic markers
Título del documento: Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and genebank collection by the neutral and functional genomic markers
Revista: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000336135
ISSN: 0717-3458
Autores: 1
1
1
1
Instituciones: 1University of Tsukuba, Graduate School of Life and Environmental Sciences, Tsukuba, Ibaraki. Japón
Año:
Periodo: Nov
Volumen: 13
Número: 6
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés A preliminary characterization was undertaken to describe genetic structure of mango ginger (Curcuma amada) acquired from farmers and ex situ genebank in Myanmar using neutral (rice SSR based RAPDs) and functional genomic (P450 based analog) markers. The high polymorphism (> 91%) depicted has displayed existence of genetic variability in the germplasm investigated. Large number of source-specific alleles (neutral-markers = 78, functional-markers = 63) was amplified which revealed that neutral regions of the mango ginger were more variable compared with the functional regions. The major fraction of the molecular variance (neutral-markers = 85%, functional-markers = 93%) was explained within germplasm acquisition sources and this tendency was also supported by the estimate of gene diversity. The genebank accessions have shown comparatively more genetic variability than farmers’ accessions. The variability observed in mango ginger may possibly be associated with the long history of its cultivation under diverse ecological conditions. The two marker systems elucidated their high resolving power which detected variability even in fewer genotypes assayed. As the target sites of these markers are different, therefore, the variability detected is believed to cover diverse part of the genome together with neutral and functional regions. We found the concurrent use of the different types of molecular markers valuable to comprehend a dependable variability pattern in the germplasm assayed
Disciplinas: Biología
Palabras clave: Angiospermas,
Genética,
Genotipos,
Diversidad genética,
Marcadores genéticos,
Bancos de germoplasma,
Curcuma amada,
Zingiberaceae
Keyword: Biology,
Angiosperms,
Genetics,
Genotypes,
Genetic diversity,
Genomic markers,
Germplasm banks,
Curcuma amada,
Zingiberaceae
Texto completo: Texto completo (Ver PDF)