| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336072 |
| ISSN: | 0717-3458 |
| Autores: | Kantachote, Duangporn1 Prachyakij, Pakorn1 Charernjiratrakul, Wilawan1 Ongsakul, Metta1 Duangjitcharoen, Yodsawee2 Chaiyasut, Chaiyavat2 Nitoda, Teruhiko3 Kanzaki, Hiroshi3 |
| Instituciones: | 1Prince of Songkhla University, Faculty of Science, Hat-Yai, Songkhla. Tailandia 2Chiang Mai University, Faculty of Pharmacy, Chiang Mai. Tailandia 3Okayama University, The Graduate School of Natural Science and Technology, Okayama. Japón |
| Año: | 2010 |
| Periodo: | Sep |
| Volumen: | 13 |
| Número: | 5 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B12 and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 109 and 1012 cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p < 0.05) for the liver weight ratio (LWR) in a group fed with 1012 cells per mouse when compared with the control group |
| Disciplinas: | Biología, Química |
| Palabras clave: | Química de alimentos, Biotecnología, Bebidas fermentadas, Contaminación microbiana, Rhodotorula mucilaginosa, Probióticos, Lactobacillus plantarum, Acido fenil-láctico |
| Keyword: | Biology, Chemistry, Food chemistry, Biotechnology, Fermented beverages, Microbial pollution, Rhodotorula mucilaginosa, Probiotics, Lactobacillus plantarum, Phenyllactic acid |
| Texto completo: | Texto completo (Ver PDF) |
