Revista: | Electronic journal of biotechnology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000357852 |
ISSN: | 0717-3458 |
Autores: | Riansa-ngawong, Wiboon1 Suwansaard, Maneewan2 Prasertsan, Poonsuk1 |
Instituciones: | 1Prince of Songkla University, Faculty of Agro-Industry, Songkhla. Tailandia 2Bowling Green State University, Department of Biological Sciences, Bowling Green, Ohio. Estados Unidos de América |
Año: | 2012 |
Volumen: | 15 |
Número: | 6 |
País: | Chile |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental |
Resumen en inglés | Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 ± 2ºC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L-1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g L-1, ethanol yield 0.47 g g-1) than those of free cells (ethanol production 10.8 g L-1, ethanol yield 0.44 g g-1) after 36 hrs of cultivation. In contrast, the small size of PPF (sPPF) was selected as a carrier in repeated batch fermentation for cost effectiveness. The ethanol productivity of immobilized yeast cells in repeated batch fermentations was 45.2-51.6% greater than that obtained from batch fermentations. The immobilized cells on sPPF improved the ethanol production and could be reused 4 times with retaining the activity of 93%. In conclusion, PPF is a potential carrier in the immobilization system. The pre-treatment of PPF increases the surface area that enhances cell adsorption and ethanol production by C. shehatae TISTR5843 |
Disciplinas: | Biología, Química |
Palabras clave: | Biotecnología, Fermentación, Sustratos experimentales, Fibra, Palma, Bioproducción, Etanol, Candida shehatae |
Keyword: | Biology, Chemistry, Fermentation, Biotechnology, Experimental substrates, Fiber, Palm, Bioproduction, Ethanol, Candida shehatae |
Texto completo: | Texto completo (Ver HTML) |