| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000398054 |
| ISSN: | 0717-3458 |
| Autores: | Zhu, Guangqin1 Chen, Qiuju1 Wang, Yunfei1 Yang, Mingming1 Song, Yuxuan1 Wang, Jiangang1 Cao, Binyun1 |
| Instituciones: | 1Northwest A&F University, College of Animal Science and Technology, Yangling, Shaanxi. China |
| Año: | 2011 |
| Volumen: | 14 |
| Número: | 3 |
| Paginación: | 1-6 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Nota breve o noticia |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap- PCR. Thus, the new approach developed in this study is simple, time - and cost -effective, and it is not limited to particular gene expression levels of each tissue |
| Disciplinas: | Biología, Química |
| Palabras clave: | Genética, Bioquímica, Clonación, ADN, Factor inhibidor de leucemia, Membrana mucosa, Mucosa oral, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Biology, Chemistry, Genetics, Biochemistry, Cloning, DNA, Leukemia inhibitory factor, Mucous membrane, Oral mucosa, Polymerase chain reaction (PCR) |
| Texto completo: | Texto completo (Ver PDF) |
