Production and biochemical characterization of xylanases synthesized by the thermophilic fungus Rasamsonia emersonii S10 by solid-state cultivation
Título del documento: Production and biochemical characterization of xylanases synthesized by the thermophilic fungus Rasamsonia emersonii S10 by solid-state cultivation
Revista: Ecletica quimica
Base de datos:
Número de sistema: 000552515
ISSN: 0100-4670
Autores: 1
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Instituciones: 1São Paulo State University, Institute of Biosciences, Languages and Exact Sciences, São José do Rio Preto, Brazil.,
2Universidad Santiago de Cali, Facultad de Ciencias Basicas, Cali, Colombia.,
Año:
Volumen: 46
Paginación: 53-67
País: Brasil
Idioma: Inglés
Resumen en inglés The xylanolytic enzyme complex hydrolyzes xylan, and these enzymes have various industrial applications. The goal of this work was to characterize the endoxylanases produced by the thermophilic fungus Rasamsonia emersonii in solid-state cultivation. Tests were carried out to evaluate the effects of pH, temperature, glycerol and phenolic compounds on enzyme activity. Thermal denaturation of one isolated enzyme was evaluated. The crude extract from R. emersonii was applied to breakdown pretreated sugarcane bagasse, by quantifying the release of xylose and glucose. The optimum pH value for the crude enzymatic extract was 5.5, and 80 °C was the optimum temperature. Regarding the stability of the crude extract, the highest values occurred between the pH ranges from 4 to 5.5. Several phenolic compounds were tested, showing an increase in enzymatic activity on the crude extract, except for tannic acid. Zymography displayed four corresponding endoxylanase bands, which were isolated by extraction from a polyacrylamide gel. The thermodynamic parameters of isolated Xylanase C were evaluated, showing a half-life greater than 6 h at 80 °C (optimum temperature), in addition to high melting temperature (93.3 °C) and structural resistance to thermal denaturation. Pretreated sugarcane bagasse breakdown by the crude enzymatic extract from R. emersonii has good hemicellulose conversion to xylose.
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