| Revista: | Brazilian journal of veterinary research and animal science |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000291612 |
| ISSN: | 1413-9596 |
| Autores: | Ferreira, Jorge Luís1 Toniolli, Ricardo Duarte, Ana Beatriz Graca Campagnari, Francine2 Boscaro, Ariadne Padovez Pazini, Fabiane Siqueira Garcia, Jose Fernando |
| Instituciones: | 1Universidade Estadual do Ceara, Laboratorio de Reproducao Suina e Tecnologia de Semen, Fortaleza, Ceara. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Laboratorio de Bioquimica e Biologia Molecular Animal, Aracatuba, Sao Paulo. Brasil |
| Año: | 2002 |
| Volumen: | 39 |
| Número: | 4 |
| Paginación: | 208-212 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The improvement of techniques to maximize reproductive potential of females needs the whole comprehension of the controlling mechanisms of follicular development. An alternative for this purpose is the quantification of relative gene expression from those genes involved in recruitment, selection and follicular development, using reverse transcriptase - polymerase chain reaction (RT - PCR). The present study aimed to quantify relative gene expression from insulin-like growth factor I (IGF-I) and follicle stimulating hormone receptor (FSHR) in cattle (Bos primigenius indicus), using as internal control the gliceraldheyde 3 phosphate dehydrogenase (GAPDH) gene. Ovaries in different estral cycle stages were obtained from slaughtered animals. Total RNA from follicles and ovarian tissue was purified and the RT-PCR conditions were standardized. PCR products were analyzed in ethydium bromide agarose gels and the bands submitted to densitometric analysis. Exponential amplification curves were constructed and the method's validation was performed using regression analysis to determine the amplification coefficient (E) for each of the three genes. Relative expression for each gene was calculated using the formula described by Prelle et al.12. In every sample there was gene expression detected for each gene showing differences related to cycle temperature. Amplification coefficient (E) was similar between control gene (GAPDH) and IGFI, independently of the class analyzed. IGFI linear amplification could be related to the constitutive character |
| Disciplinas: | Medicina veterinaria y zootecnia, Química |
| Palabras clave: | Bovinos, Reproducción y genética animal, Bioquímica, Semi-cuantitativo de RT-PCR, Expresión génica, Ganado bovino |
| Keyword: | Veterinary medicine and animal husbandry, Chemistry, Animal reproduction and genetics, Bovines, Biochemistry, Semi-quantitative RT-PCR, Gene expression, Cattle |
| Texto completo: | Texto completo (Ver HTML) |
