| Revista: | Brazilian journal of physical therapy |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000376018 |
| ISSN: | 1809-9246 |
| Autores: | Souza, Nadhia H.C1 Ferrari, Raquel A.M1 Silva, Daniela F.T3 Nunes, Fabio D4 Bussadori, Sandra K2 Fernandes, Kristianne P.S2 |
| Instituciones: | 1Universidade Nove de Julho, Programa de Pos-graduacao em Ciencias da Reabilitacao, Sao Paulo. Brasil 2Universidade Nove de Julho, Programa de Pos-graduacao em Biofotonica Aplicada as Ciencias da Saude, Sao Paulo. Brasil 3Universidade Nove de Julho, Departamento de Ciencias Exatas, Sao Paulo. Brasil 4Universidade de Sao Paulo, Faculdade de Odontologia, Sao Paulo. Brasil |
| Año: | 2014 |
| Periodo: | Jul-Ago |
| Volumen: | 18 |
| Número: | 4 |
| Paginación: | 303-314 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Macrophages play a major role among the inflammatory cells that invade muscle tissue following an injury. Low-level laser therapy (LLLT) has long been used in clinical practice to accelerate the muscle repair process. However, little is known regarding its effect on macrophages. Objective: This study evaluated the effect of LLLT on the mitochondrial activity (MA) of macrophages. Method: J774 macrophages were treated with lipopolysaccharide (LPS) and interferon – gamma (IFN-γ) (activation) for 24 h to simulate an inflammatory process, then irradiated with LLLT using two sets of parameters (780 nm; 70 mW; 3 J/cm2 and 660 nm; 15 mW; 7.5 J/cm2). Non-activated/nonirradiated cells composed the control group. MA was evaluated by the cell mitochondrial activity (MTT) assay (after 1, 3 and 5 days) in three independent experiments. The data were analyzed statistically. Results: After 1 day of culture, activated and 780 nm irradiated macrophages showed lower MA than activated macrophages, but activated and 660 nm irradiated macrophages showed MA similar to activated cells. After 3 days, activated and irradiated (660 nm and 780 nm) macrophages showed greater MA than activated macrophages, and after 5 days, the activated and irradiated (660 nm and 780 nm) macrophages showed similar MA to the activated macrophages. Conclusions: These results show that 660 nm and 780 nm LLLT can modulate the cellular activation status of macrophages in inflammation, highlighting the importance of this resource and of the correct determination of its parameters in the repair process of skeletal muscle |
| Disciplinas: | Medicina |
| Palabras clave: | Terapéutica y rehabilitación, Traumatología, Reparación muscular, Laser de baja energía, Actividad mitocondrial, Macrófagos |
| Keyword: | Medicine, Therapeutics and rehabilitation, Traumatology, Muscle repair, Low energy laser, Mitochondrial activity, Macrophages |
| Texto completo: | Texto completo (Ver PDF) |
