Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects
Título del documento: Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451857
ISSN: 1984-8250
Autores: 1
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Instituciones: 1Zhejiang University of Technology, College of Pharmaceutical Science, Zhejiang, Hangzhou. China
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Serrapeptase, a proteolytic enzyme, has been used for the adjuvant treatment of many diseases. However, its fibrinolytic activity is still uncertain. Herein, the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects were investigated. The results showed that the fibrinolytic activity of serrapeptase was 1295 U/mg, and the specific activity was 3867 U/mg of protein when its proteolytic activity toward casein was 2800 U/mg. The optimum temperature and pH for serrapeptase activity were 37-40°C and 9.0, respectively. At 1 mmol/L, Zn2+, Mn2+ and Fe2+ could activate the fibrinolytic activity of serrapeptase, while K+, Cu2+, sodium dodecyl sulfate (SDS) and ethylene diamine tetraacetic acid (EDTA) inhibited it. In vitro tests showed that serrapeptase could completely prevent blood coagulation at 150 U/mL, and the percentage of blood clot lysis reached 96.6% at 37°C after 4 h at 300 U/mL. These results indicate that serrapeptase has excellent fibrinolytic activity, and can be used as a health food or candidate drug for the prevention or treatment of thrombotic diseases
Disciplinas: Medicina
Palabras clave: Sistema cardiovascular,
Farmacología,
Proteasas,
Serrapeptasa,
Fibrinólisis
Keyword: Cardiovascular system,
Pharmacology,
Proteases,
Serrapeptase,
Fibrinolysis
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