| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451753 |
| ISSN: | 1984-8250 |
| Autores: | Wang, Min1 Wang, Shan1 Zhang, Yi1 Liu, Huimin1 Li, Pengyue1 Du, Shouying1 |
| Instituciones: | 1Beijing University of Chinese Medicine, School of Chinese Materia Medica, Beijing. China |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Xingnaojing (XNJ) injection was used to treat pneumonia and stroke in clinic in China, but with poor patient compliance. Xingnaojing nanoemulsion for intranasal delivery was developed to improve it. This article tried to evaluate the mucosal irritation of Xingnaojing nanoemulsion and investigate cellular uptake mechanism of its encapsulated lipophilic drugs. The toad palate model and rat nasal mucosa model were used to study the nasal ciliotoxicity and nasal mucosal irritation of nanoemulsion to evaluate its safety intranasally. The cellular uptake mechanism was studied by Calu-3 cell model. Coumarin 6 was encapsulated in nanoemulsion and the endocytic pathways were studied by cellular uptake experiments after being treated with different inhibitors. In toad palate model, the cilia movement of Xingnaojing nanoemulsion group last for 467.40 ± 39.02 min, which was obviously longer than deoxycholate group (90.60 ± 15.40 min). Studies on rats showed that the damage caused by nanemulsion is capable of being recovered. Nanoemulsion uptake was reduced obviously when cells were treated with wortmannin, and it also decreased about 13% when the temperature reduced from 37ºC to 4ºC. Mucosal irritation caused by nanoemulsion is low and the damage is recoverable. The cellular uptake of Xingnaojing nanoemulsion is energy-dependent, and macropinocytosis was the most important pathway for cellular uptake |
| Disciplinas: | Medicina |
| Palabras clave: | Neumología, Farmacología, Medicina tradicional, Nanoemulsiones, Ciliotoxicidad nasal, Endocitosis, Micropinocitosis |
| Keyword: | Pneumology, Pharmacology, Folk medicine, Nanoemulsions, Nasal ciliotoxicity, Endocytosis, Micropinocytosis |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
