| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451929 |
| ISSN: | 1984-8250 |
| Autores: | Gomes, Lorena Caixeta1 Resende, Rodrigo Ribeiro1 Parreira, Ricardo Cambraia1 Ferreira, Cláudia Natália1 Reis, Edna Afonso2 Duarte, Rita Carolina Figueiredo1 Alves, Luan Carlos Vieira1 Araújo, Sergio Schusterschitz da Silva3 Carvalho, Maria das Graças1 Sabino, Adriano de Paula1 |
| Instituciones: | 1Universidade Federal de Minas Gerais, Faculdade de Farmacia, Belo Horizonte, Minas Gerais. Brasil 2Universidade Federal de Minas Gerais, Instituto de Ciencias Exatas, Belo Horizonte, Minas Gerais. Brasil 3Universidade Federal de Minas Gerais, Hospital de Clinicas,, Belo Horizonte, Minas Gerais. Brasil |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The present study evaluated 56 patients diagnosed with Chronic Lymphocytic Leukemia (CLL) and a control group of 44 clinically healthy subjects with no previous history of leukemia. Genetic expressions of AKT and microRNAs were evaluated by quantitative PCR (qPCR). A significant increase in AKT gene expression in patients when compared to controls was observed (p = 0.017). When the patients were stratified according to Binet subgroups, a significant difference was observed between the subgroups, with this protein kinase appearing more expressed in the B+C subgroup (p = 0.013). Regarding miRNA expression, miR-let-7b and miR-26a were reduced in CLL patients, when compared to controls. However, no significant differences were observed in these microRNA expressions between the Binet subgroups (A versus B+C). By contrast, miR-21 to miR-27a oncogenes showed no expression difference between CLL patients and controls. AKT protein kinase is involved in the signaling cascade that occurs with BCR receptor activation, leading to increased lymphocyte survival and protection against the induction of cell death in CLL. Thus, increased AKT protein kinase expression and the reduction of miR-let-7b and miR-26a, both tumor suppressors, may explain increased lymphocyte survival in CLL patients and may be promising markers for the prognostic evaluation of this disease |
| Disciplinas: | Medicina |
| Palabras clave: | Oncología, Hematología, Genética, Leucemia linfocítica crónica, Patogénesis, Expresión génica, MicroARNs, AKT proteína cinasa, Apoptosis |
| Keyword: | Oncology, Hematology, Genetics, Chronic lymphocytic leukemia, Pathogenesis, Gene expression, AKT protein kinase, MicroRNAs, Apoptosis |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
