| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452122 |
| ISSN: | 1984-8250 |
| Autores: | Rotondo, Rosana1 Cruz, Pablo Santa2 Masin, Marianela3 Bürgi, Milagros4 Girardini, Javier3 García, Stella M1 Rodríguez, Gustavo R1 Furlan, Ricardo L. E2 Escalante, Andrea M2 |
| Instituciones: | 1Universidad Nacional de Rosario, Facultad de Ciencias Agronómicas, Zavalla, Santa Fe. Argentina 2Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmaceúticas, Rosario, Santa Fe. Argentina 3Universidad Nacional de Rosario, Instituto de Biología Molecular de Rosario, Rosario, Santa Fe. Argentina 4Universidad Nacional del Litoral, Facultad de Bioquímica y Ciencias Biológicas, Santa Fe. Argentina |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The aim of this work is to study three cultivars of artichoke (Cynara cardunculus var. scolymus): Gauchito, Guri and Oro Verde in terms of their in vitro chemoprevention and anti-inflammatory properties. These cultivars show good productive performance. The phenolic composition of their fresh leaves and edible bracts was analyzed by high performance liquid chromatography and high resolution mass spectrometry (HPLC-HRMS), showing mainly caffeoylquinic acids and flavonoids. Caffeoylquinic acids were quantified and the highest content was found in Gauchito cultivar. In this cultivar, the content of dicaffeoylquinic acids in fresh bracts was six times higher than that in fresh leaves (10064.5 ± 378.3 mg/kg versus 1451.0 ± 209.3 mg/kg respectively). Luteolin flavonoids were detected in leaves. The extracts from fresh bracts and leaves were assessed in their in vitro bioactivity against human neuroblastoma cells (SH-SY5Y). Inhibition of SH-SY5Y cells proliferation by Gauchito and Guri leaf extracts (8 µg/mL) was higher than 50 %. The leaf extracts of the same cultivars showed an inhibitory effect on human interferon IFN-I, decreasing its activity 50% at 40 µg/mL. Interestingly, the bract extracts did not show in vitro bioactivity at these concentrations, nor did the pure compounds chlorogenic acid, cynarin, apigenin and luteolin (at 2 µg/mL). These results suggest that Gauchito and Guri leaf extracts have potential for human neuroblastoma chemoprevention and treatment of inflammatory processes |
| Disciplinas: | Biología, Química |
| Palabras clave: | Angiospermas, Fitoquímica, Alcachofa, Compuestos fenólicos, Actividad antiinflamatoria, Neuroblastoma, Cynara cardunculus, Asteraceae |
| Keyword: | Angiosperms, Phytochemistry, Artichoke, Phenolic compounds, Antiinflammatory activity, Neuroblastoma, Cynara cardunculus, Asteraceae |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
