Anti urolithiatic activity of Cyperus rotundus tubers: In silico, In vitro and In vivo approaches
Título del documento: Anti urolithiatic activity of Cyperus rotundus tubers: In silico, In vitro and In vivo approaches
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451830
ISSN: 1984-8250
Autores: 1
2
1
1
1
Instituciones: 1Creative Educational Society’s College of Pharmacy, Kurnool, Andhra Pradesh. India
2Santhiram College of Pharmacy, Nandyal, Andhra Pradesh. India
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The present research evaluated the anti urolithic potential of Cyperus rotundus tubers extract using in silico, in vitro and in vivo techniques. In silicostudy was performed of Cyperus rotundus constituents and pathological protein oxalate oxidase (PDB Id: 2ETE). In vitrostudy, nucleation and aggregation assay involved for assessment of ethanol extract of Cyperus rotundus tuber (50-3000 µg/ml).In vivo studies involved that the Cyperus rotundusethanolic extract (100, 200 and 400 mg/kg B.wt.) wastreatedonsodium oxalate induced urolithiatic rats for seven days,evaluated kidney function by urine and serum biochemical analysis and statistical analysis performed usingGraphPad prism5 software.In silico results showedthat Cyperus rotundus constituents,Humulene epoxide, 4-Oxo-alpha-ylangene, Cubebol were exhibited better binding energyonoxalate oxidase.Ethanolic extract of Cyperus rotundustuber was exhibited nucleation, aggregation of calcium oxalate monohydrate crystals inhibition in dosedependent manner. Sodium oxalate treatment was triggered biochemical changesin the urine that have been substantially prevented by the ethanolic extract of Cyperus rotundus tuber. The current findings Cyperus rotundus anti urolithic activity due to antioxidant essential oils. The molecular docking results could be used to optimize lead and develop the appropriate urolithiasis treatment
Disciplinas: Medicina
Palabras clave: Nefrología,
Farmacología,
Urolitiasis,
Plantas medicinales,
Nucleación,
Oxalato oxidasa,
Oxalato de sodio,
Cyperus rotundus,
Cyperaceae
Keyword: Nephrology,
Pharmacology,
Urolithiasis,
Medicinal plants,
Nucleation,
Oxalate oxidase,
Sodium oxalate,
Cyperus rotundus,
Cyperaceae
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