| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308716 |
| ISSN: | 0104-6632 |
| Autores: | Calsavara, L.P.V1 Moraes, F.F Zanin, G.M |
| Instituciones: | 1Universidade Estadual de Maringa, Departamento de Engenharia Quimica, Maringa, Parana. Brasil |
| Año: | 2000 |
| Periodo: | Dic |
| Volumen: | 17 |
| Número: | 4-7 |
| Paginación: | 841-848 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Commercial cellobiase has been immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67% and 13.7%, respectively. Thermal stability of the free and immobilized enzyme (IE) was determined with 0.2% w/v cellobiose solution, pH 4.8, temperatures from 40 to 70°C for free enzyme and 40 to 75°C for IE. Free cellobiase maintained its activity practically constant for 240 min at temperatures up to 55°C. The IE has shown higher stability retaining its activity in the same test up to 60° C. Half-lives for free enzyme were 14.1, 2.1 and 0.17 h at 60, 65 and 70°C, respectively, whereas the IE at the same temperatures had half-lives of 245, 21.3 and 2.9 h. The energy of thermal deactivation was 80.6 kcal/mol for the free enzyme and 85.2 kcal/mol for the IE, confirming stabilization by immobilization |
| Disciplinas: | Química |
| Palabras clave: | Ingeniería química, Biotecnología, Enzimas inmovilizadas, Celobiosa, Estabilidad térmica |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Immobilized enzymes, Cellobiose, Thermal stability |
| Texto completo: | Texto completo (Ver HTML) |
