| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308719 |
| ISSN: | 0104-6632 |
| Autores: | Bassetti, F.J1 Bergamasco, R2 Moraes, F.F Zanin, G.M |
| Instituciones: | 1Centro Federal de Educacao Tecnologica, Campo Mourao, Parana. Brasil 2Universidade Estadual de Maringa, Departamento de Engenharia Quimica, Maringa, Parana. Brasil |
| Año: | 2000 |
| Periodo: | Dic |
| Volumen: | 17 |
| Número: | 4-7 |
| Paginación: | 867-872 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The thermal stability and the energy of deactivation of free invertase and the immobilized enzyme (IE) was measured at temperatures in the range of 35 to 65°C for the hydrolysis of a 5% w/v sucrose solution. The free enzyme at pH 5.0 is stable up to 50°C for a period of 4 h. Invertase immobilized in controlled pore silica by the silane-glutaraldehyde covalent method is stable up to 55ºC, in pH 4.5 for the same period. For higher temperatures the enzyme deactivation follows the exponential decay model and half-lives are 0.53, 1.80, and 13.9 h for free invertase, at 65, 60, and 55ºC, respectively. For the IE half-lives are 0.48, 1.83, and 20.9 h, at 65, 60, and 55ºC, respectively. The IE is more stable than the free invertase; the energy of deactivation being 83.1 kcal/mol for the IE and 72.0 kcal/mol for the free enzyme |
| Disciplinas: | Química |
| Palabras clave: | Ingeniería química, Biotecnología, Enzimas inmovilizadas, Invertasa, Sacarosa, Estabilidad térmica, Energía de deactivación |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Immobilized enzymes, Invertase, Sucrose, Thermal stability, Deactivation energy |
| Texto completo: | Texto completo (Ver HTML) |
