| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308636 |
| ISSN: | 0104-6632 |
| Autores: | Minami, N.M1 Lucarini, A.C2 Kilikian, B.V |
| Instituciones: | 1Universidade de Sao Paulo, Escola Politecnica, Sao Paulo. Brasil 2Faculdade de Engenharia Industrial, Departamento de Engenharia Quimica, Sao Bernardo do Campo, Sao Paulo. Brasil |
| Año: | 1999 |
| Periodo: | Jun |
| Volumen: | 16 |
| Número: | 2 |
| Paginación: | 193-198 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa |
| Disciplinas: | Química |
| Palabras clave: | Ingeniería química, Biotecnología, Cromatografía por filtración en gel, Glucoamilasa, Cultivo sumergido, Cultivo semisólido, Purificación, Aspergillus awamori |
| Keyword: | Chemistry, Biotechnology, Chemical engineering, Gel filtration chromatography, Glucoamylase, Submerse culture, Semisolid culture, Aspergillus awamori, Purification |
| Texto completo: | Texto completo (Ver HTML) |
