Aprotinin recovery: comparison between biospecific and pseudobiospecific affinity adsorptions



Título del documento: Aprotinin recovery: comparison between biospecific and pseudobiospecific affinity adsorptions
Revista: Brazilian journal of chemical engineering
Base de datos: PERIÓDICA
Número de sistema: 000308653
ISSN: 0104-6632
Autores: 1


2
Instituciones: 1Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil
2Universite Technologie de Compiegne, Centre de Recherches de Royallieu, Compiegne, Oise. Francia
Año:
Periodo: Jun
Volumen: 16
Número: 2
Paginación: 119-127
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Two adsorption techniques were studied as potential methods for the recovery of aprotinin: a biospecific adsorption (using immobilized trypsin and chymotrypsin as ligants) and a pseudobiospecific adsorption of aprotinin-trypsin complex onto IMAC matrix. These studies indicated that ionic strength has an important effect on aprotinin adsorption on immobilized trypsin, and the pH was the most significant variable in aprotinin desorption from both immobilized enzymes. The aprotinin-trypsin complex adsorption onto the IMAC matrix was not as significantly affected by changes in pH as it was for changes in ionic strength. For the desorption step, both variables significantly affected the complex recovery. However, the effect of ionic strength was markedly stronger for this desorption step
Disciplinas: Química
Palabras clave: Química farmacéutica,
Aprotinina,
Recuperación de proteínas,
Adsorción por afinidad
Keyword: Chemistry,
Medicinal chemistry,
Aprotinin,
Protein recovery,
Affinity adsorption
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