| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000374047 |
| ISSN: | 1516-8913 |
| Autores: | Bresolin, Iara Rocha Antunes Pereira1 Bresolin, Igor Tadeu Lazzarotto2 Silveira, Edgar3 Tambourgi, Elias Basile1 Mazzola, Priscila Gava4 |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil 2Universidade Federal de Sao Paulo, Departamento de Ciencias Exatas e da Terra, Diadema, Sao Paulo. Brasil 3Universidade Federal de Uberlandia, Instituto de Genetica e Bioquimica, Uberlandia, Minas Gerais. Brasil 4Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil |
| Año: | 2013 |
| Periodo: | Nov-Dic |
| Volumen: | 56 |
| Número: | 6 |
| Paginación: | 971-979 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity |
| Disciplinas: | Biología, Química |
| Palabras clave: | Angiospermas, Fitoquímica, Bromelaína, Extracción química, Purificación química, Actividad enzimática, Piña |
| Keyword: | Biology, Chemistry, Angiosperms, Phytochemistry, Bromelain, Chemical extraction, Chemical purification, Enzymatic activity, Pineapple |
| Texto completo: | Texto completo (Ver HTML) |
