Isolation and mycelial growth of Diehliomyces microsporus: effect of culture medium and incubation temperature



Título del documento: Isolation and mycelial growth of Diehliomyces microsporus: effect of culture medium and incubation temperature
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000348398
ISSN: 1516-8913
Autores: 1
2
Instituciones: 1Universidade Federal de Pelotas, Instituto de Biologia, Pelotas, Rio Grande do Sul. Brasil
2Universidade Estadual Paulista "Julio de Mesquita Filho", Departamento de Producao Vegetal, Botucatu, Sao Paulo. Brasil
Año:
Periodo: Jul
Volumen: 50
Número: 4
Paginación: 587-595
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés The false truffle is one of the main problems in the production of the Agaricus brasiliensis in Brazil and the control of this fungal competitor has been rather difficult due to difficulties in the isolation and cultivation of this pathogen. This experiment was conducted in three stages, the first consisting of the isolation of Diehliomyces microsporus starting from portions of the fruiting body and through the ascospores suspension; second, D. microsporus cultivated in vitro at 15, 20, 25, 30 and 35oC in six different culture media (CSDA, OCDA, PCDA, ODA, PDA, CDA); third, D. microsporus was inoculated on sterilized compost for formation of the fruiting body. The colony formation from tissue of D. microsporus starting from portions of fruiting body was more efficient than germination of the ascospores. Compost medium (CDA) allowed a larger diameter of the D. microsporus colony, followed by the medium made up of compost and potato mixture, favoring a denser composition. The largest mycelial growth speed of D. microsporus occurred when the culture was incubated at 28 and 30oC. Incubation temperatures lower than 15oC or above 35oC inhibited the mycelial growth of D. microsporus completely. The fruiting bodies were obtained easily in sterilized compost and later inoculated along with mycelial competitor
Disciplinas: Química,
Biología,
Agrociencias
Palabras clave: Bioquímica,
Hongos,
Fitopatología,
Fitotecnia,
Agaricus brasiliensis,
Setas,
Trufas falsas,
Medios de cultivo,
Diehliomyces microsporus
Keyword: Chemistry,
Biology,
Agricultural sciences,
Biochemistry,
Fungi,
Crop husbandry,
Phytopathology,
Agaricus brasiliensis,
Mushrooms,
False truffles,
Culture media,
Diehliomyces microsporus
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