| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000387720 |
| ISSN: | 1516-8913 |
| Autores: | Mohansrinivasan, V1 Devi, Subathra1 Deori, Meenakshi1 Biswas, Ananya1 Naine, Jemimah1 |
| Instituciones: | 1VIT University, School of Biosciences and Technology, Vellore, Tamil Nadu. India |
| Año: | 2015 |
| Periodo: | Jul-Ago |
| Volumen: | 58 |
| Número: | 4 |
| Paginación: | 540-546 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate, ethyl-9- cis -11- trans octadecadienoate and (R)-(-)-14,-methyl-8-Hexadecyn-1-ol in petroleum ether extract. Cytotoxic activity of the GSE was observed against skin cancer cell lines A4321 using 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide) MTT assay. The IC50 value of the GSE against A431 skin cancer cell line was 480 µg/mL. This is first such report against A4321 cell lines. The study gives the overall perception about importance of GSE in medicine and nutraceuticals purposes |
| Disciplinas: | Medicina, Química |
| Palabras clave: | Farmacología, Medicina experimental, Oncología, Fitoquímica, Extractos vegetales, Semillas, Uva, Cáncer, Piel |
| Keyword: | Medicine, Chemistry, Experimental medicine, Oncology, Pharmacology, Phytochemistry, Plant extracts, Seeds, Grape, Cancer, Skin |
| Texto completo: | Texto completo (Ver HTML) |
