Revista: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000337633 |
ISSN: | 1516-8913 |
Autores: | Thomaz-Soccol, Andréa1 Mocellin, Marcos1 Mulinari, Fabiane1 Castro, Edilene Alcântara de2 Queiroz-Telles, Flávio de3 Alcântara, Fernanda de Souza2 Bavaresco, Marcello Tortelli3 Hennig, Luciane3 Andraus, Anielo3 Luz, Ennio3 Thomaz-Soccol, Vanete2 |
Instituciones: | 1Universidade Federal do Parana, Hospital das Clinicas, Londrina, Parana. Brasil 2Universidade Federal do Parana, Departamento de Patologia Basica, Londrina, Parana. Brasil 3Universidade Federal do Parana, Setor de Ciencias da Saude, Londrina, Parana. Brasil |
Año: | 2011 |
Periodo: | May-Jun |
Volumen: | 54 |
Número: | 3 |
Paginación: | 487-494 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Analítico |
Resumen en inglés | The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture, which allowed isolating and identifying the parasite and – with the PCR technique - it was possible to diagnose 100% MCL patients for all the three repetitions of exams. The PCR optimized for the present work proved to be an auxiliary method for diagnosing leishmaniasis applicable in the patients carrying MCL due to Leishmania (Viannia) braziliensis and did not need culture to be performed, resulting in a faster diagnosis |
Disciplinas: | Medicina |
Palabras clave: | Diagnóstico, Protozoarios, Parasitología, Mucocutaneous leishmaniasis, Biología molecular, Leishmania braziliensis, Brasil |
Keyword: | Medicine, Diagnosis, Public health, Parasitology, Mucocutaneous leishmaniasis, Molecular biology, Leishmania braziliensis, Brazil, Protozoa |
Texto completo: | Texto completo (Ver HTML) |