| Revista: | Biotecnología aplicada |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000349275 |
| ISSN: | 0864-4551 |
| Autores: | Morris, Humberto J1 Carrillo, Olimpia B2 Almarales, Angel3 Bermudez, Rosa C1 Alonso, María E2 Borges, Leonardo4 Quintana, María M4 Fontainer, Roberto1 Llauradó, Gabriel1 Hernández, Martha5 |
| Instituciones: | 1Universidad de Oriente, Centro de Estudios de Biotecnología Industrial, Santiago de Cuba. Cuba 2Universidad de La Habana, Facultad de Biología, La Habana. Cuba 3Centro de Aplicaciones Tecnológicas para el Desarrollo Sostenible, Guantánamo. Cuba 4Ministerio de Ciencia, Tecnología y Medio Ambiente, Centro de Investigaciones en Energía Solar, Santiago de Cuba. Cuba 5Universidad de Ciego de Avila, Centro de Bioplantas, Ciego de Avila. Cuba |
| Año: | 2009 |
| Volumen: | 26 |
| Número: | 2 |
| Paginación: | 162-165 |
| País: | Cuba |
| Idioma: | Inglés |
| Tipo de documento: | Reporte técnico |
| Enfoque: | Experimental |
| Resumen en inglés | Chlorella vulgaris (Chlorophyta, Chlorophyceae) has received a particular attention in the programmes of microalgae utilisation in biotechnology. Enzymatic hydrolysis of cell proteins represents a very promising method to increase protein digestibility and thus, for obtaining hydrolysates with improved nutritional and functional properties. However, this technology has been little approached and the biological evaluation of hydrolysates has had a strictly nutritional nature. The design of hydrolysis conditions that combined for the first time, the use of C.vulgaris 87/1 treated with ethanol and pancreatin at pH values of 7.5-8.0, led to a product with a degree of hydrolysis of 20-22% and yields of 50-55%, characterised by a high digestibility (97.2%) and nitrogen solubility over a wide pH range (2.0- 10.0). Hydrolysis curves were fitted to an exponential model, common to many food proteins. The bulk of the product dry matter consists of soluble peptides and free amino acids (47.7%) with three main peptides of molecular masses between 2 and 5 kDa. The oral administration of Chlorella hydrolysate (500 mg/kg) to undernourished Balb/c mice provided benefits in terms of liver protein metabolism and the induction of anabolic processes in gut mucosa. The hydrolysate also enhanced the immunological recovery, as judged by the stimulation of haemopoiesis, monocytemacrophage system activation, as well as humoral and cell mediated immune functions, like T-dependent antibody response and the reconstitution of delayed-type hypersensitivity (DTH) response. These results represent the first findings in the world concerning the immunomodulating effects of a microalgae protein hydrolysate |
| Disciplinas: | Química, Biología, Medicina |
| Palabras clave: | Química de alimentos, Algas, Metabolismo y nutrición, Hidrolizados proteicos, Chlorella vulgaris, Inmunonutrición |
| Keyword: | Chemistry, Biology, Medicine, Food chemistry, Algae, Metabolism and nutrition, Protein hydrolysate, Chlorella vulgaris, Immunonutrition |
| Texto completo: | Texto completo (Ver PDF) |
