| Revista: | Anais da Academia Brasileira de Ciencias |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000420580 |
| ISSN: | 0001-3765 |
| Autores: | Costa, Gessica A1 Rossatto, Fernanda C.P1 Medeiros, Aline W1 Correa, Ana Paula F2 Brandelli, Adriano2 Frazzon, Ana Paula G1 Motta, Amanda de S. da1 |
| Instituciones: | 1Universidade Federal do Rio Grande do Sul, Instituto de Ciencias Basicas da Saude, Porto Alegre, Rio Grande do Sul. Brasil 2Universidade Federal do Rio Grande do Sul, Instituto de Ciencia e Tecnologia de Alimentos, Porto Alegre, Rio Grande do Sul. Brasil |
| Año: | 2018 |
| Periodo: | Mar |
| Volumen: | 90 |
| Número: | 1 |
| Paginación: | 73-84 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure |
| Disciplinas: | Química |
| Palabras clave: | Química de alimentos, Microbiología, Biopelículas, Adhesión bacteriana, Industria alimentaria, Péptidos antibacterianos, Bioensayos, Actividad antibacteriana, Staphylococcus aureus, Enterococcus faecalis |
| Keyword: | Food chemistry, Microbiology, Biofilms, Bacterial adhesion, Food industry, Antibacterial peptides, Bioassays, Antibacterial activity, Staphylococcus aureus, Enterococcus faecalis |
| Texto completo: | Texto completo (Ver HTML) |
