Physiological and biochemical changes during desiccation tolerance loss in millet (Pennisetum glaucum L.) seeds
Título del documento: Physiological and biochemical changes during desiccation tolerance loss in millet (Pennisetum glaucum L.) seeds
Revista: Acta scientiarum. Agronomy
Base de datos: PERIÓDICA
Número de sistema: 000460117
ISSN: 1679-9275
Autores:
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Instituciones: 1Universidade Federal de Lavras, Departamento de Agricultura, Lavras, Minas Gerais. Brasil
Año:
Volumen: 41
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés The aim of this study was to evaluate the physiological and biochemical changes related to desiccation tolerance loss in millet seeds. The studied points of the germination process were determined according to the seed imbibition curve of the millet hybrid ADRF6010: control (0h), 3h of imbibition, 1 and 3 mm radicle. The seeds were dried on silica gel for 72h at 20°C, followed by pre-humidification at 25°C for 24h. Seed physiological quality was evaluated by electrical conductivity and a germination test, and seed vigor was evaluated with a first germination count and a germination speed index. The experiment was performed in a completely randomized design, and means were compared by the Scott-Knott test at a 5% probability. The enzymatic systems of superoxide dismutase, catalase, peroxidase, and α-amylase, as well as the expression of heat-resistant proteins were evaluated. Enzymatic activity was quantified with the ImageJ® software. Millet seeds lost desiccation tolerance when the radicle reached 1 mm in length. According to enzymatic standards, peroxidase and α-amylase activity, as well as heat-resistant protein activity, were related to desiccation tolerance loss in millet seeds
Disciplinas: Agrociencias,
Biología,
Química
Palabras clave: Fitoquímica,
Gramíneas,
Fisiología vegetal,
Mijo,
Pennisetum glaucum,
Desecación,
Germinación,
Proteínas,
Enzimas
Keyword: Gramineae,
Plant physiology,
Phytochemistry,
Millet,
Pennisetum glaucum,
Desiccation,
Germination,
Proteins,
Enzymes
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