| Revista: | Acta pediátrica de México |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000402101 |
| ISSN: | 0186-2391 |
| Autores: | Flores Pérez, Angélica1 Gayosso Vázquez, Catalina2 Alcántar Curiel, María Dolores2 Arzate Barbosa, Patricia3 Alpuche Aranda, Celia4 Arredondo García, José Luis1 |
| Instituciones: | 1Instituto Nacional de Pediatría, Laboratorio de Investigación Clínica, México, Distrito Federal. México 2Universidad Nacional Autónoma de México, Facultad de Medicina, México, Distrito Federal. México 3Instituto Nacional de Pediatría, Laboratorio de Bacteriología, México, Distrito Federal. México 4Instituto Nacional de Salud Pública, Centro de Investigación en Enfermedades Infecciosas, Cuernavaca, Morelos. México |
| Año: | 2015 |
| Periodo: | Sep-Oct |
| Volumen: | 36 |
| Número: | 5 |
| Paginación: | 383-388 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Enteric bacteria causing nosocomial infections are often resistant to third-generation cephalosporins due to the production of extended-spectrum β-lactamases (ESBLs). Objective: To describe and characterize the ESBLs pattern present in Klebsiella pneumoniae and Serratia marcescens strains, isolated as causative of nosocomial sepsis in pediatric patients at Instituto Nacional de Pediatría (National Institute of Pediatrics). Material and methods: We analyzed 94 strains of K. pneumoniae and 7 of S. marcescens isolated from clinical specimens from 2002-2005, causative of sepsis in a children's hospital. We evaluated antibiotic susceptibility and detection of ESBL phenotypes by disk diffusion methods; ceftazidime-resistant isolates were further characterized by pulsed field gel electrophoresis (PFGE); and ESBLs were phenotypically and genotypically characterized by isoelectric focusing, polymerase chain reaction (PCR) and sequencing. We also assed for presence of conjuga-tive plasmids bearing the ESBL gene. Results: 51/94 (54%) of K. pneumoniae isolates, and 5/7 (71%) of S. marcescens isolates were resistant to ceftazidime; all carried a blaSHV5 gene. All K. pneumoniae isolates had a distinct PFGE profile, yet all carried a -48-Kb plasmid, that was conjugatively transferable to an Escherichia coli receptor, which expressed the resistance phenotype. On the other hand, all S. marcescens isolates had a similar PFGE profile, were unable to transfer the ceftazidime-resistance phenotype, and were isolated from the same ward in a short time-span suggesting an outbreak. Conclusions: The overall prevalence of ESBL-producing enteric bacteria in this hospital is high but similar to other Latin American reports. The sulfhydryl variable-5 (SHV-5) ESBL gene appears to reside in a highly mobile plasmid, capable of spreading among different K. pneumoniae clones and perhaps even to S. marcescens |
| Disciplinas: | Medicina |
| Palabras clave: | Farmacología, Pediatría, Bacterias, Infección hospitalaria, Resistencia bacteriana, Beta-lactamasa, Klebsiella pneumoniae, Serratia marcescens |
| Keyword: | Medicine, Pediatrics, Pharmacology, Bacteria, Nosocomial infections, Bacterial resistance, Beta-lactamase, Klebsiella pneumoniae, Serratia marcescens |
| Texto completo: | Texto completo (Ver HTML) |
